A simple, accurate and validated liquid chromatographic method was developed for the estimation of rifampicin (RIF) and isoniazid (INH) in bulk and combined dosage forms. Drugs were injected in a chromatographic system on a reverse-phase C8 column using a mobile phase composition of Acetonitrile: Ammonium acetate buffer (50:50), isocratically and monitored at the analogous maximum of each compound. Peaks were recognized with a retention time as compared with standards and established with characteristic spectra using the PDA detector. The concentrations of the solutions were measured on a weight base to eliminate the use of an internal standard. The method was found linear (r 2 = 0.999 for RIF and 0.998 for INH), precise (RSD%: 1.56 for RIF and 0.82 for INH), accurate (overall average recovery yields: 100.1% for RIF and 100.59% for INH) and selective. Retention time (min) for RIF and INH was found 3.8 and 2.3 respectively. Because of simplicity and accuracy, the method is appropriate for regular quality control analysis of anti-tuberculosis bulk drug and dosage form. The proposed method of HPLC was developed and validated thoroughly for the quantitative analysis of RIF and INH in bulk and combined dosage form. The method was found to be simple, rapid, accurate, precise and economical and gives an acceptable recovery of the analytes, which can be directly and quickly applied for routine analysis in quality control of RIF and INH mixture in pharmaceutical bulk and formulations.
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